Reverse Electron Transfer by Respiratory Complex I Catalyzed in a Modular Proteoliposome System.

ABSTRACT: Respiratory complex I is an essential metabolic enzyme that uses the energy from NADH oxidation and ubiquinone reduction to translocate protons across an energy transducing membrane and generate the proton motive force for ATP synthesis. Under specific conditions, complex I can also catalyze the reverse reaction, Δp-linked oxidation of ubiquinol to reduce NAD⁺ (or O₂), known as reverse electron transfer (RET). Oxidative damage by reactive oxygen species generated during RET underpins ischemia reperfusion injury, but as RET relies on several converging metabolic pathways, little is known about its mechanism or regulation. Here, we demonstrate Δp-linked RET through complex I in a synthetic proteoliposome system for the first time, enabling complete kinetic characterizat

SUBMITTER: Wright JJ 

PROVIDER: S-EPMC9026280 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature