Transcriptomic Analysis of E. coli after Exposure to a Sublethal Concentration of Hydrogen Peroxide Revealed a Coordinated Up-Regulation of the Cysteine Biosynthesis Pathway.

ABSTRACT: Hydrogen peroxide (H₂O₂) is a key defense component of host-microbe interaction. However, H₂O₂ concentrations generated by immune cells or epithelia are usually insufficient for bacterial killing and rather modulate bacterial responses. Here, we investigated the impact of sublethal H₂O₂ concentration on gene expression of E. coli BW25113 after 10 and 60 min of exposure. RNA-seq analysis revealed that approximately 12% of bacterial genes were strongly dysregulated 10 min following exposure to 2.5 mM H₂O₂. H₂O₂ exposure led to the activation of a specific antioxidant response and a general stress response. The latter was characterized by a transient down-regulation of genes in

SUBMITTER: Roth M 

PROVIDER: S-EPMC9026346 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature