Association between effector-type regulatory T cells and immune checkpoint expression on CD8⁺ T cells in malignant ascites from epithelial ovarian cancer.

ABSTRACT:

Background

Regulatory T cells (Tregs) play an important role in the antitumor immune response in epithelial ovarian cancer (EOC). To understand the immune-inhibitory networks of EOC, we addressed the association between Tregs and immune checkpoint expression on T cells in the tumor microenvironment of EOC.

Methods

A total of 41 patients with stage IIIC and IV EOC were included in the analysis. We harvested cells from malignant ascites and investigated them using multi-color flow cytometry. We categorized the Tregs into 3 groups: effector-type Tregs, naïve Tregs and non-Tregs, based on the expression patterns of CD45RA and Foxp3 in CD4⁺ T cells. Furthermore, the relationships between the expression of various immune checkpoint molecules, such as PD-1, on CD8^+<

SUBMITTER: Sato S 

PROVIDER: S-EPMC9026673 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature