Multi-Layered Human Blood Vessels-on-Chip Design Using Double Viscous Finger Patterning.

ABSTRACT: Blood vessel-on-a-chip models aim at reproducing vascular functions. However, very few efficient methods have been designed to address the need for biological replicates in medium- to high-throughput screenings. Here, vessels-on-chip were designed in polydimethylsiloxane-glass chips using the viscous finger patterning technique which was adapted to create channels with various internal diameters inside a collagen solution and to simultaneously seed cells. This method was refined to create blood vessels composed of two concentric, distinct, and closely appositioned layers of human endothelial and perivascular cells arranged around a hollow lumen. These approaches allowed the formation of structurally correct blood vessels-on-chips which were constituted of either only endothelial cells or o

SUBMITTER: Delannoy E 

PROVIDER: S-EPMC9027030 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature