Contribution of Oxidative Stress Induced by Sonodynamic Therapy to the Calcium Homeostasis Imbalance Enhances Macrophage Infiltration in Glioma Cells.

ABSTRACT:

Background

To better understand the Ca²⁺ overload mechanism of SDT killing gliomas, we examined the hypothesis that the early application of the mechanosensitive Ca²⁺ channel Piezo1 antagonist (GsMTx4) could have a better anti-tumor effect.

Methods

The in vitro effect of low-energy SDT combined with GsMTx4 or agonist Yoda 1 on both the ROS-induced distribution of Ca²⁺ as well as on the opening of Piezo1 and the dissociation and polymerization of the Ca²⁺ lipid complex were assessed. The same groups were also studied to determine their effects on both tumor-bearing BALB/c-nude and C57BL/6 intracranial tumors, and their effects on the tumor-infiltrating macrophages were studied as well.

Results

It was determined that ultrasoun

SUBMITTER: Chen L 

PROVIDER: S-EPMC9027216 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature