Isolation and Characterisation of Bacteriophage Selective for Key <i>Acinetobacter baumannii</i> Capsule Chemotypes.

ABSTRACT: Nineteen bacteriophages against five main capsular types of multidrug-resistant Acinetobacter baumannii were isolated from tertiary care hospital sewage. Eight representative phages from each capsular type were characterized and tested for their biological properties. The biological features revealed that phages T1245, T444, and T515 had a large burst size of more than 420 pfu/mL, together with a short latent period lasting less than 6 min, and were readily adsorbed to a bacterial host within 10 min. Moreover, these phages demonstrated host specificity and stability over a broad range of temperatures (-20 to 60 °C) and pH (5.0-9.0). A whole-genome analysis of six lytic and two temperate phages revealed high genomic similarity with double-stranded DNA between 40 and 50 kb and G + C c

SUBMITTER: Soontarach R 

PROVIDER: S-EPMC9027227 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature