Circulating cell free DNA and citrullinated histone H3 as useful biomarkers of NETosis in endometrial cancer.

ABSTRACT:

Background

Cancer mortality is mainly caused by organ failure and thrombotic events. It has been demonstrated that NETosis, a chromatin release mechanism implemented by neutrophils, may contribute to these lethal systemic effects. Our aim was to investigate NETosis biomarkers in endometrial cancer (EC).

Methods

The experiments were conducted on 21 healthy subjects (HS) with no gynecological conditions, and on 63 EC patients. To assess the presence of NETosis features, IHC and IF was performed using antibodies against citrullinated histone H3 (citH3), neutrophil elastase (NE) and histone 2B. Serum levels of cell free DNA (cfDNA), cell free mitochondrial DNA (cfmtDNA) and citH3 were measured by qPCR using one microliter of deactivated serum, and by ELISA assay respectively. Fr

SUBMITTER: Ronchetti L 

PROVIDER: S-EPMC9027343 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature