Dynamic Distribution of ASIC1a Channels and Other Proteins within Cells Detected through Fractionation.

ABSTRACT: Proteins in eukaryotic cells reside in different cell compartments. Many studies require the specific localization of proteins and the detection of any dynamic changes in intracellular protein distribution. There are several methods available for this purpose that rely on the fractionation of the different cell compartments. Fractionation protocols have evolved since the first use of a centrifuge to isolate organelles. In this study, we described a simple method that involves the use of a tabletop centrifuge and different detergents to obtain cell fractions enriched in cytosolic (Cyt), plasma membrane (PM), membranous organelle (MO), and nuclear (Nu) proteins and identify the proteins in each fraction. This method serves to identify transmembrane proteins such as channel subunits as well a

SUBMITTER: Salinas Castellanos LC 

PROVIDER: S-EPMC9027401 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature