Hop Tannins as Multifunctional Tyrosinase Inhibitor: Structure Characterization, Inhibition Activity, and Mechanism.

ABSTRACT: The application of hops could be extended to obtain higher commercial values. Tannins from hops were assessed for their tyrosinase inhibition ability, and the associated mechanisms were explored. Nuclear magnetic resonance (NMR) and high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry (HPLC-ESI-MS/MS) revealed that the hop tannins were characterized as condensed tannins with (epi)catechin and (epi)gallocatechin as subunits and an average polymerization degree of 10.32. Tyrosinase inhibition assay indicated that hop tannins had an IC₅₀ = 76.52 ± 6.56 μM. Kinetic studies of the inhibition processes indicated the tannins provided inhibition through competitive-uncompetitive mixed reactions. In silico molecule docking showed that tannins were bound

SUBMITTER: Liu J 

PROVIDER: S-EPMC9027561 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature