Engineered Glycosidases for the Synthesis of Analogs of Human Milk Oligosaccharides.

ABSTRACT: Enzymatic synthesis is an elegant biocompatible approach to complex compounds such as human milk oligosaccharides (HMOs). These compounds are vital for healthy neonatal development with a positive impact on the immune system. Although HMOs may be prepared by glycosyltransferases, this pathway is often complicated by the high price of sugar nucleotides, stringent substrate specificity, and low enzyme stability. Engineered glycosidases (EC 3.2.1) represent a good synthetic alternative, especially if variations in the substrate structure are desired. Site-directed mutagenesis can improve the synthetic process with higher yields and/or increased reaction selectivity. So far, the synthesis of human milk oligosaccharides by glycosidases has mostly been limited to analytical reactions with mass s

SUBMITTER: Nekvasilova P 

PROVIDER: S-EPMC9027921 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature