Diminished LC3-Associated Phagocytosis by Huntington's Disease Striatal Astrocytes.

ABSTRACT:

Background

In recent years the functions of astrocytes have shifted from conventional supportive roles to also include active roles in altering synapses and engulfment of cellular debris. Recent studies have implicated astrocytes in both protective and pathogenic roles impacting Huntington's disease (HD) progression.

Objective

The goal of this study is to determine if phagocytosis of cellular debris is compromised in HD striatal astrocytes.

Methods

Primary adult astrocytes were derived from two HD mouse models; the fast-progressing R6/2 and slower progressing Q175. With the use of laser nanosurgery, a single astrocyte was lysed within an astrocyte network. The phagocytic response of astrocytes was observed with phase contrast and by fluorescence microscopy for GFP-LC3

SUBMITTER: Wakida NM 

PROVIDER: S-EPMC9028675 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature