Carbon Nanotube-Mediated Plasmid DNA Delivery in Rice Leaves and Seeds.

ABSTRACT: CRISPR-Cas gene editing technologies offer the potential to modify crops precisely; however, in vitro plant transformation and regeneration techniques present a bottleneck due to the lengthy and genotype-specific tissue culture process. Ideally, in planta transformation can bypass tissue culture and directly lead to transformed plants, but efficient in planta delivery and transformation remains a challenge. This study investigates transformation methods that have the potential to directly alter germline cells, eliminating the challenge of in vitro plant regeneration. Recent studies have demonstrated that carbon nanotubes (CNTs) loaded with plasmid DNA can diffuse through plant cell walls, facilitating transient expression of foreign genetic elements in plant tissues. To test if this approa

SUBMITTER: Dunbar T 

PROVIDER: S-EPMC9028948 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature