A Biomimetic Porcine Urothelial Model for Assessing Escherichia coli Pathogenicity.

ABSTRACT: Urinary tract infections can be severe, sometimes fatal, diseases whose etiological pathogens are predominantly uropathogenic strains of E. coli (UPEC). To investigate the UPEC pathogenesis, several models have already been established with minor or major disadvantages. The aim was to develop a simple, fast, and inexpensive biomimetic in vitro model based on normal porcine urothelial (NPU) cells that are genetically and physiologically similar to human bladder urothelium and to perform basic studies of E. coli pathogenicity. Initially, the model was tested using a set of control E. coli strains and, subsequently, with human E. coli strains isolated either from patients with urinary infections or from the feces of healthy individuals. A drop in viability of NPU c

SUBMITTER: Predojevic L 

PROVIDER: S-EPMC9029248 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature