Distinctive High Expression of Antiretroviral APOBEC3 Protein in Mouse Germinal Center B Cells.

ABSTRACT: Tissue and subcellular localization and its changes upon cell activation of virus-restricting APOBEC3 at protein levels are important to understanding physiological functions of this cytidine deaminase, but have not been thoroughly analyzed in vivo. To precisely follow the possible activation-induced changes in expression levels of APOBEC3 protein in different mouse tissues and cell populations, genome editing was utilized to establish knock-in mice that express APOBEC3 protein with an in-frame FLAG tag. Flow cytometry and immunohistochemical analyses were performed prior to and after an immunological stimulation. Cultured B cells expressed higher levels of APOBEC3 protein than T cells. All differentiation and activation stages of freshly prepared B cells expressed significant levels of AP

SUBMITTER: Tsukimoto S 

PROVIDER: S-EPMC9029289 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature