TSA Activates Pluripotency Factors in Porcine Recloned Embryos.

ABSTRACT: Animal cloning is of great importance to the production of transgenic and genome-edited livestock. Especially for multiple gene-editing operations, recloning is one of the most feasible methods for livestock. In addition, a multiple-round cloning method is practically necessary for animal molecular breeding. However, cloning efficiency remains extremely low, especially for serial cloning, which seriously impedes the development of livestock breeding based on genome editing technology. The incomplete reprogramming and failure in oocyte activation of some pluripotent factors were deemed to be the main reason for the low efficiency of animal recloning. Here, to overcome this issue, which occurred frequently in the process of animal recloning, we established a reporter system in which fluoresc

SUBMITTER: Feng T 

PROVIDER: S-EPMC9029504 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature