Radiation-Induced Bystander Effect Mediated by Exosomes Involves the Replication Stress in Recipient Cells.

ABSTRACT: Exosomes released by irradiated cells mediate the radiation-induced bystander effect, which is manifested by DNA breaks detected in recipient cells; yet, the specific mechanism responsible for the generation of chromosome lesions remains unclear. In this study, naive FaDu head and neck cancer cells were stimulated with exosomes released by irradiated (a single 2 Gy dose) or mock-irradiated cells. Maximum accumulation of gamma H2A.X foci, a marker of DNA breaks, was detected after one hour of stimulation with exosomes from irradiated donors, the level of which was comparable to the one observed in directly irradiated cells (a weaker wave of the gamma H2A.X foci accumulation was also noted after 23 h of stimulation). Exosomes from irradiated cells, but not from control ones, activated two st

SUBMITTER: Smolarz M 

PROVIDER: S-EPMC9029583 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature