Determination of Brain Tissue Samples Storage Conditions for Reproducible Intraoperative Lipid Profiling.

ABSTRACT: Ex-vivo molecular profiling has recently emerged as a promising method for intraoperative tissue identification, especially in neurosurgery. The short-term storage of resected samples at room temperature is proposed to have negligible influence on the lipid molecular profiles. However, a detailed investigation of short-term molecular profile stability is required to implement molecular profiling in a clinic. This study evaluates the effect of storage media, temperature, and washing solution to determine conditions that provide stable and reproducible molecular profiles, with the help of ambient ionization mass spectrometry using rat cerebral cortex as model brain tissue samples. Utilizing normal saline for sample storage and washing media shows a positive effect on the reproducibility of t

SUBMITTER: Pekov SI 

PROVIDER: S-EPMC9029908 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature