N-1 Perfusion Platform Development Using a Capacitance Probe for Biomanufacturing.

ABSTRACT: Fed-batch process intensification with a significantly shorter culture duration or higher titer for monoclonal antibody (mAb) production by Chinese hamster ovary (CHO) cells can be achieved by implementing perfusion operation at the N-1 stage for biomanufacturing. N-1 perfusion seed with much higher final viable cell density (VCD) than a conventional N-1 batch seed can be used to significantly increase the inoculation VCD for the subsequent fed-batch production (referred as N stage), which results in a shorter cell growth phase, higher peak VCD, or higher titer. In this report, we incorporated a process analytical technology (PAT) tool into our N-1 perfusion platform, using an in-line capacitance probe to automatically adjust the perfusion rate based on real-time VCD measurements. The capa

SUBMITTER: Rittershaus ESC 

PROVIDER: S-EPMC9029935 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature