Downregulation of Squalene Synthase Broadly Impacts Isoprenoid Biosynthesis in Guayule.

ABSTRACT: Production of natural rubber by Parthenium argentaum (guayule) requires increased yield for economic sustainability. An RNAi gene silencing strategy was used to engineer isoprenoid biosynthesis by downregulation of squalene synthase (SQS), such that the pool of farnesyl diphosphate (FPP) substrate might instead be available to initiate natural rubber synthesis. Downregulation of SQS resulted in significantly reduced squalene and slightly increased rubber, but not in the same tissues nor to the same extent, partially due to an apparent negative feedback regulatory mechanism that downregulated mevalonate pathway isoprenoid production, presumably associated with excess geranyl pyrophosphate levels. A detailed metabolomics analysis of isoprenoid production in guayule revealed significan

SUBMITTER: Placido D 

PROVIDER: S-EPMC9030042 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature