Methylglyoxal Impairs Sister Chromatid Separation in Lymphocytes.

ABSTRACT: The accurate segregation of sister chromatids is complex, and errors that arise throughout this process can drive chromosomal instability and tumorigenesis. We recently showed that methylglyoxal (MGO), a glycolytic by-product, can cause chromosome missegregation events in lymphocytes. However, the underlying mechanisms of this were not explored. Therefore, in this study, we utilised shotgun proteomics to identify MGO-modified proteins, and label-free quantitation to measure changes in protein abundance following exposure to MGO. We identified numerous mitotic proteins that were modified by MGO, including those involved in the separation and cohesion of sister chromatids. Furthermore, the protein abundance of Securin, an inhibitor of sister chromatid separation, was increased following trea

SUBMITTER: Donnellan L 

PROVIDER: S-EPMC9030103 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature