Comprehensive Characterization of Platelet-Enriched MicroRNAs as Biomarkers of Platelet Activation.

ABSTRACT: Dysregulation of platelet function is causally connected to thrombus formation and cardiovascular diseases. Therefore, assessing platelet reactivity is crucial. However, current platelet function tests come with pitfalls, limiting clinical use. Plasma miRNA signatures have been suggested as novel biomarkers for predicting/diagnosing cardiovascular diseases and monitoring antiplatelet therapy. Here, we provide results from a comprehensive study on the feasibility of using circulatory platelet miRNAs as surrogate markers of platelet activation. We performed small RNA-Seq on different blood cell types to confirm known and identify novel platelet-enriched miRNAs and validated a panel of 16 miRNAs using RT-qPCR. To identify the main carrier of these blood-based platelet miRNAs, we enriched and

SUBMITTER: Krammer TL 

PROVIDER: S-EPMC9030873 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature