Peptide Bond Formation between Aminoacyl-Minihelices by a Scaffold Derived from the Peptidyl Transferase Center.

ABSTRACT: The peptidyl transferase center (PTC) in the ribosome is composed of two symmetrically arranged tRNA-like units that contribute to peptide bond formation. We prepared units of the PTC components with putative tRNA-like structure and attempted to obtain peptide bond formation between aminoacyl-minihelices (primordial tRNAs, the structures composed of a coaxial stack of the acceptor stem on the T-stem of tRNA). One of the components of the PTC, P1c2^UGGU (74-mer), formed a dimer and a peptide bond was formed between two aminoacyl-minihelices tethered by the dimeric P1c2^UGGU. Peptide synthesis depended on both the existence of the dimeric P1c2^UGGU and the sequence complementarity between the ACCA-3' sequence of the minihelix. Thus, the tRNA-like structures deri

SUBMITTER: Kawabata M 

PROVIDER: S-EPMC9030986 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature