Quantitative Estimation of Synergistic Toxicity of Cu and Zn on Growth of Arabidopsis thaliana by Isobolographic Method.

ABSTRACT: Heavy metal is one of the most frequent soil contaminants and contaminated soils generally include numerous metals. Although exposure to multiple metals may increase the toxicity to humans and ecosystems, only additive effects are considered in the risk assessment. In this study, the synergistic effect of heavy metals (Cu and Zn) on a model plant, Arabidopsis thaliana, was quantified by the isobolographic method. The plant was cultured via the growth assay method on a plant agar containing individual heavy metals or combinations of Cu + Zn in a growth chamber. The concentration of Cu varied by eight levels from 0 to 200 μM and the concentration of Zn also varied by eight levels from 0 to 400 μM. In the combination of metals, each of the three levels of Cu (25-75 μM) and Zn (20-100 μ

SUBMITTER: Bae B 

PROVIDER: S-EPMC9031100 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature