The Novel-miR-659/SPP1 Interaction Regulates Fat Deposition in Castrated Male Pigs.

ABSTRACT: Castration is usually used to remove boar taint in commercial pork production, but the adipose accumulation was increased excessively, which affected the meat quality of pigs. Based on our previous study, secreted phosphoprotein 1 (SPP1) was significantly differentially expressed between castrated and intact male pigs. However, the role of SPP1 in regulating adipose growth and fat storage caused by castration is unknown. In this study, SPP1 was identified to inhibit adipogenesis by the expression of adipogenic markers PPARγ and FABP4 as well as Oil red staining assay during differentiation of porcine bone marrow mesenchymal stem cells (pBMSCs). Subsequently, testosterone was used to treat pBMSCs to simulate the androgen status of intact pigs. Compared wit

SUBMITTER: Xiao L 

PROVIDER: S-EPMC9031235 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature