Scanning Electrochemical Microscopy-Somatic Cell Count as a Method for Diagnosis of Bovine Mastitis.

ABSTRACT: The method to diagnose mastitis is generally the somatic cell count (SCC) by flow cytometry measurement. When the number of somatic cells in raw milk is 2.0 × 10⁵ cells/mL or more, the condition is referred to as mastitis. In the current study, we created a milk cell chip that serves as an electrochemical method that can be easily produced and used utilizing scanning electrochemical microscopy (SECM). The microelectrode present in the cell chip scans, and the difference between the oxygen concentration near the milk cell chip and in bulk is measured as the oxygen (O₂) reduction current. We estimated the relationship between respiratory activity and the number of somatic cells in raw milk as a calibration curve, using scanning electrochemical microscopy-somatic cell co

SUBMITTER: Kasai S 

PROVIDER: S-EPMC9031417 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature