Frequent Spindle Assembly Errors Require Structural Rearrangement to Complete Meiosis in Zea mays.

ABSTRACT: The success of an organism is contingent upon its ability to faithfully pass on its genetic material. In the meiosis of many species, the process of chromosome segregation requires that bipolar spindles be formed without the aid of dedicated microtubule organizing centers, such as centrosomes. Here, we describe detailed analyses of acentrosomal spindle assembly and disassembly in time-lapse images, from live meiotic cells of Zea mays. Microtubules organized on the nuclear envelope with a perinuclear ring structure until nuclear envelope breakdown, at which point microtubules began bundling into a bipolar form. However, the process and timing of spindle assembly was highly variable, with frequent assembly errors in both meiosis I and II. Approximately 61% of cells formed incorrect sp

SUBMITTER: Weiss JD 

PROVIDER: S-EPMC9031645 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature