Low-Cost Platform for Multiplexed Electrochemical Melting Curve Analysis.

ABSTRACT: Detection and identification of single nucleotide polymorphisms (SNPs) have garnered increasing interest in the past decade, finding potential application in detection of antibiotic resistance, advanced forensic science, as well as clinical diagnostics and prognostics, moving toward the realization of personalized medicine. Many different techniques have been developed for genotyping SNPs, and ideally these techniques should be rapid, easy-to-use, cost-effective, flexible, scalable, easily automated, and requiring minimal end-user intervention. While high-resolution melting curve analysis has been widely used for the detection of SNPs, fluorescence detection does not meet many of the desired requirements, and electrochemical detection is an attractive alternative due to its high sensitivit

SUBMITTER: Chahin N 

PROVIDER: S-EPMC9031717 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature