Live-Cell Imaging of the Contractile Velocity and Transient Intracellular Ca²⁺ Fluctuations in Human Stem Cell-Derived Cardiomyocytes.

ABSTRACT: Live-cell imaging techniques are essential for acquiring vital physiological and pathophysiological knowledge to understand and treat heart disease. For live-cell imaging of transient alterations of [Ca²⁺]_i in human cardiomyocytes, we engineered human-induced pluripotent stem cells carrying a genetically-encoded Ca²⁺-indicator (GECI). To monitor sarcomere shortening and relaxation in cardiomyocytes in real-time, we generated a α-cardiac actinin (ACTN2)-copepod (cop) green fluorescent protein (GFP⁺)-human-induced pluripotent stem cell line by using the CRISPR-Cas9 and a homology directed recombination approach. The engineered human-induced pluripotent stem cells were differentiated in transgenic GECI-enhanced GFP⁺-cardiomyocytes and ACT

SUBMITTER: Acharya A 

PROVIDER: S-EPMC9031802 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature