Preclinical Development of Bioengineered Allografts Derived from Decellularized Human Diaphragm.

ABSTRACT: Volumetric muscle loss (VML) is the traumatic/surgical loss of skeletal muscle, causing aesthetic damage and functional impairment. Suboptimal current surgical treatments are driving research towards the development of optimised regenerative therapies. The grafting of bioengineered scaffolds derived from decellularized skeletal muscle may be a valid option to promote structural and functional healing. In this work, a cellular human diaphragm was considered as a scaffold material for VML treatment. Decellularization occurred through four detergent-enzymatic protocols involving (1) sodium dodecyl sulfate (SDS), (2) SDS + Tergitol^TM, (3) sodium deoxycholate, and (4) Tergitol^TM. After decellularization, cells, DNA (≤50 ng/mg of tissue), and muscle fibres were efficiently

SUBMITTER: Barbon S 

PROVIDER: S-EPMC9031975 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature