Extracellular Vesicles Derived from SIPA1^high Breast Cancer Cells Enhance Macrophage Infiltration and Cancer Metastasis through Myosin-9.

ABSTRACT: Tumour cell metastasis can be genetically regulated by proteins contained in cancer cell-derived extracellular vesicles (EVs) released to the tumour microenvironment. Here, we found that the number of infiltrated macrophages was positively correlated with the expression of signal-induced proliferation-associated 1 (SIPA1) in invasive breast ductal carcinoma tissues and MDA-MB-231 xenograft tumours. EVs derived from MDA-MB-231 cells (231-EVs) significantly enhanced macrophage migration, compared with that from SIPA1-knockdown MDA-MB-231 cells (231/si-EVs) both in vitro and in vivo. We revealed that SIPA1 promoted the transcription of MYH9, which encodes myosin-9, and up-regulated the expression level of myosin-9 in breast cancer cells and their EVs. We also found that blocking

SUBMITTER: Feng L 

PROVIDER: S-EPMC9032110 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature