Fluid-Phase Endocytosis and Lysosomal Degradation of Bovine Lactoferrin in Lung Cells.

ABSTRACT: The iron-binding protein lactoferrin and the cell-penetrating peptides derived from its sequence utilise endocytosis to enter different cell types. The full-length protein has been extensively investigated as a potential therapeutic against a range of pathogenic bacteria, fungi, and viruses, including SARS-CoV-2. As a respiratory antiviral agent, several activity mechanisms have been demonstrated for lactoferrin, at the extracellular and plasma membrane levels, but as a protein that enters cells it may also have intracellular antiviral activity. Characterisation of lactoferrin's binding, endocytic traffic to lysosomes, or recycling endosomes for exocytosis is lacking, especially in lung cell models. Here, we use confocal microscopy, flow cytometry, and degradation assays to evaluate bindin

SUBMITTER: Sayers EJ 

PROVIDER: S-EPMC9032238 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature