Revisiting the Resazurin-Based Sensing of Cellular Viability: Widening the Application Horizon.

ABSTRACT: Since 1991, the NAD(P)H-aided conversion of resazurin to fluorescent resorufin has been widely used to measure viability based on the metabolic activity in mammalian cell culture and primary cells. However, different research groups have used divergent assay protocols, scarcely reporting the systematic optimization of the assay. Here, we perform extensive studies to fine-tune the experimental protocols utilizing resazurin-based viability sensing. Specifically, we focus on (A) optimization of the assay dynamic range in individual cell lines for the correct measurement of cytostatic and cytotoxic properties of the compounds; (B) dependence of the dynamic range on the physical quantity detected (fluorescence intensity versus change of absorbance spectrum); (C) calibration of the assay for the

SUBMITTER: Lavogina D 

PROVIDER: S-EPMC9032648 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature