Evidence-based selection of reference genes for RT-qPCR assays in periodontal research.

ABSTRACT:

Objective

To underline the necessity of adequate reference genes for real-time quantitative polymerase chain reaction (RT-qPCR) and evaluate a novel tool for condition-specific reference gene selection.

Background

RT-qPCR is a commonly used experimental technique that allows for highly sensitive analysis of gene transcription. Moreover, the use of internal reference genes as a means for relative quantification has rendered RT-qPCR a straightforward method for a variety of sciences, including dentistry. However, the expressional stability of internal reference genes must be evaluated for every assay in order to account for possible quantification bias.

Materials and methods

Herein, we used the software tool RefGenes to identify putatively stable reference genes with th

SUBMITTER: Diehl D 

PROVIDER: S-EPMC9033546 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature