Ultrasensitive biosensing platform based on luminescence quenching ability of fullerenol quantum dots.

ABSTRACT: An ultrasensitive biosensing platform for DNA and ochratoxin A (OTA) detection is constructed based on the luminescence quenching ability of fullerenol quantum dots (FOQDs) for the first time. As the surface of FOQDs is largely covered by hydroxyl groups, stable colloidal suspension of FOQDS in aqueous solution can be obtained, which is very advantageous for application in biosensing compared to nano-C₆₀. FOQDs can effectively quench the fluorescence of dyes with different emission wavelengths that are tagged to bioprobes to an extent of more than 87% in aqueous buffer solution through a PET mechanism. Moreover, the nonspecific quenching of the fluorescent dyes (not bound to bioprobes) caused by FOQDs is negligible, so the background signal is extremely low which is beneficial f

SUBMITTER: Li H 

PROVIDER: S-EPMC9033561 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature