Aggresome assembly at the centrosome is driven by CP110-CEP97-CEP290 and centriolar satellites.

ABSTRACT: Protein degradation is critical to maintaining cellular homeostasis, and perturbation of the ubiquitin proteasome system leads to the accumulation of protein aggregates. These aggregates are either directed towards autophagy for destruction or sequestered into an inclusion, termed the aggresome, at the centrosome. Utilizing high-resolution quantitative analysis, here, we define aggresome assembly at the centrosome in human cells. Centriolar satellites are proteinaceous granules implicated in the trafficking of proteins to the centrosome. During aggresome assembly, satellites were required for the growth of the aggresomal structure from an initial ring of phosphorylated HSP27 deposited around the centrioles. The seeding of this phosphorylated HSP27 ring depended on the centrosomal proteins

SUBMITTER: Prosser SL 

PROVIDER: S-EPMC9033585 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature