Investigation of the correlation between immune thrombocytopenia and T cell activity-regulated gene polymorphism using functional study.

ABSTRACT: Thrombocytopenia is a condition where the platelet count is under 100 × 10⁹/L, which is caused by various disorders. However, the mechanism of thrombocytopenia is still unclear. Hence, we tried to investigate the correlation between immune thrombocytopenia (ITP) and single nucleotide polymorphisms (SNPs) of genes related to T cell activation. There were 32 ITP patients and 30 healthy controls enrolled in this study. PCR and sequencing were used to find out the significant SNPs, which we focused on the promoter region of CTLA4 and CD28. In this study, the ITP cases were divided into primary ITP group, secondary ITP group, and the combination of the two to the follow-up analysis. Moreover, dual-luciferase reporter assay was used to evaluate the transcription activity of the signif

SUBMITTER: Chen DP 

PROVIDER: S-EPMC9033768 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature