Characterization of transcriptome diversity and in vitro behavior of primary human high-risk breast cells.

ABSTRACT: Biology and transcriptomes of non-cancerous human mammary epithelial cells at risk for breast cancer development were explored following primary isolation utilizing conditional reprogramming cell technology from mastectomy tissue ipsilateral to invasive breast cancer. Cultures demonstrated consistent categorizable behaviors. Relative viability and mammosphere formation differed between samples but were stable across three different mammary-specific media. E2F cell cycle target genes expression levels were positively correlated with viability and advancing age was inversely associated. Estrogen growth response was associated with Tissue necrosis factor signaling and Interferon alpha response gene enrichment. Neoadjuvant chemotherapy exposure significantly altered transcriptomes, shifting th

SUBMITTER: Alothman SJ 

PROVIDER: S-EPMC9033878 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature