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Antibacterial activity of antimicrobial peptide gcDefb1 against foodborne pathogenic bacteria and its application in pork storage.

ABSTRACT: Pork and its products are preferably contaminated by bacteria; thus, it is essential to develop low-cost, high-efficiency and biologically safe preservatives to prevent the growth of bacteria during storage. In the current study, grass carp β-defensin 1 (gcDefb1) was produced and purified from Pichia pastoris through the heterologous expression method. The in vitro antimicrobial assay demonstrated that yeast-derived gcDefb1 possesses a broad antibacterial spectrum, including both Gram-positive and -negative bacteria, and the MIC values against Escherichia coli ATCC 25,922 were as low as 30 μg/mL and showed no cytotoxicity or hemolytic activity. The bactericidal rate of gcDefb1 was less than 60 min by disrupting the cell membranes, and it inhibited the formation of bacterial biofilms. Moreover, gcDefb1 was used as a biopreservative for pork storage, indicating that the physicochemical and sensory qualities were improved. This study provides an efficient method to prepare and utilize gcDefb1 as a novel biopreservative.

Supplementary information

The online version contains supplementary material available at 10.1007/s10068-022-01060-9.

SUBMITTER: Dong B

PROVIDER: S-EPMC9033914 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature

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Antibacterial activity of antimicrobial peptide gcDefb1 against foodborne pathogenic bacteria and its application in pork storage.

Dong Bin B Lin Yanjun Y Wang Jun J Du Wen W Sun Chunlong C Fu Shijun S Wu Tao T

Food science and biotechnology 20220305 5

Pork and its products are preferably contaminated by bacteria; thus, it is essential to develop low-cost, high-efficiency and biologically safe preservatives to prevent the growth of bacteria during storage. In the current study, grass carp β-defensin 1 (gcDefb1) was produced and purified from <i>Pichia pastoris</i> through the heterologous expression method. The in vitro antimicrobial assay demonstrated that yeast-derived gcDefb1 possesses a broad antibacterial spectrum, including both Gram-pos ...[more]

PMID: 35529682

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Project description:Silver nanoparticles plays a vital role in the development of new antimicrobial substances against a number of pathogenic microorganisms. These nanoparticles due to their smaller size could be very effective as they can improve the antibacterial activity through lysis of bacterial cell wall. Green synthesis of metal nanoparticles using various plants and plant products has recently been successfully accomplished. However, few studies have investigated the use of industrial waste materials in nanoparticle synthesis. In the present investigation, synthesis of silver nanoparticles (AgNPs) was attempted using the aqueous extract of corn leaf waste of Zea mays, which is a waste material from the corn industry. The synthesized AgNPs were evaluated for their antibacterial activity against foodborne pathogenic bacteria (Bacillus cereus ATCC 13061, Listeria monocytogenes ATCC 19115, Staphylococcus aureus ATCC 49444, Escherichia coli ATCC 43890, and Salmonella Typhimurium ATCC 43174) along with the study of its synergistic antibacterial activity. The anticandidal activity of AgNPs were evaluated against Candida species (C. albicans KACC 30003 and KACC 30062, C. glabrata KBNO6P00368, C. geochares KACC 30061, and C. saitoana KACC 41238), together with the antioxidant potential. The biosynthesized AgNPs were characterized by UV-Vis spectrophotometry with surface plasmon resonance at 450 nm followed by the analysis using scanning electron microscope, X-ray diffraction, Fourier-transform infrared spectroscopy and thermogravimetric analysis. The AgNPs displayed moderate antibacterial activity (9.26-11.57 mm inhibition zone) against all five foodborne pathogenic bacteria. When AgNPs were mixed with standard antibacterial or anticandidal agent, they displayed strong synergistic antibacterial (10.62-12.80 mm inhibition zones) and anticandidal activity (11.43-14.33 mm inhibition zones). In addition, the AgNPs exhibited strong antioxidant potential. The overall results highlighted the potential use of maize industrial waste materials in the synthesis of AgNPs and their utilization in various applications particularly as antibacterial substance in food packaging, food preservation to protect against various dreadful foodborne pathogenic bacteria together with its biomedical, pharmaceutical based activities.

Project description:BackgroundThe present study dealt with the screening of soil bacteria with antibacterial activity from different locations in Bangalore, India. Antibiotics play the role of self-defense mechanism for the bacteria and are produced as secondary metabolites to protect themselves from other competitive microorganisms. The need for new antibiotics arose as the pathogenic bacteria acquire resistance to various antibiotics meant for treating human diseases. Given the importance of antibiotics of bacterial origin, standard techniques have been used to isolate and characterize the soil bacteria which showed antibacterial activity.ResultsThe isolated bacteria were tested against human pathogenic bacteria like Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, and Klebsiella pneumoniae by primary and secondary screening methods. The isolates PR1, PR2, and PR3 were confirmed to have antibacterial activity against S. aureus, E. coli, P. aeruginosa, and K. pneumoniae by both methods. Studies on the effect of filter sterilization, autoclaving, and proteinase K treatment on culture filtrates showed filter sterilization as the best method. The effect of different carbon and nitrogen sources on the antibacterial activity showed that preference by each isolate differed for carbon and nitrogen requirements. The isolates PR1, PR2, and PR3 were identified as Bacillus aryabhattai strain PR-D07, Arthrobacter humicola strain PR-F07, and Neomicrococcus lactis strain PR-F11 through 16S rRNA sequencing.ConclusionFindings from this research work are encouraging and could proceed further to applied aspects. Only 3 bacterial isolates out of 263 isolates from soil samples displayed antibacterial activity against human pathogens S. aureus, E. coli, P. aeruginosa, and K. pneumoniae. They were identified as B. aryabhattai, A. humicola, and N. lactis by 16S rRNA studies and all of them are Gram-positive. Each isolate preferred different carbon and nitrogen sources for their enhanced antibacterial activity. Efficacy of the culture filtrates of these isolates was tested by filter sterilization, autoclaving, and proteinase K treatment. Filter-sterilized culture filtrates showed higher antibacterial activity than other treatments. A comparison of the antibacterial activity of culture filtrates and antibiotic streptomycin produced an inhibition zone of 18.5 mm and 15.5 mm respectively. This is the first report on the antibacterial activity of all the 3 bacterial strains (B. aryabhattai strain PR-D07, A. humicola strain PR-F07, and N. lactis strain PR-F11), against all the human pathogens, mentioned earlier. It is also found that the antibiotic factor is proteinaceous as proteinase K considerably reduced the antibacterial activity of the culture filtrates. With the above significant results, these 3 bacteria are considered to be promising candidates for the isolation of new antibacterial agents.

Dataset Information

Antibacterial activity of antimicrobial peptide gcDefb1 against foodborne pathogenic bacteria and its application in pork storage.

Supplementary information

Publications

Antibacterial activity of antimicrobial peptide gcDefb1 against foodborne pathogenic bacteria and its application in pork storage.

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