Dataset Information

Optimization of biomass production by autochthonous Pseudomonas sp. MT1A3 as strategy to apply bioremediation in situ in a chronically hydrocarbon-contaminated soil.

ABSTRACT: These days, petroleum hydrocarbon pollution has become a global problem, because of this, bioremediation is presented as a strategy for cleaning up sites contaminated with organic pollutants, and it has an increasing role in relation to the potential it presents as a non-invasive and cost-effective technology. The aim of this study is to optimize the biomass production of Pseudomonas sp. MT1A3 strain as a soil bioremediation approach for petroleum hydrocarbon polluted environments. Factorial experimental designs were employed to study the effect of several factors of composition medium and incubation conditions on biomass production. Agro-industrial wastes such as peanut oil as carbon source, NaNO₃ as nitrogen source and incubation temperature were found to be significant independent variables. These factors were further optimized using Box-Behnken design. Combination of peanut oil 18.69 g/L, NaNO₃ 2.39 g/L and 26.06 °C incubation temperature was optimum for maximum biomass production of MT1A3 and the model validated in a bioreactor allowed to obtain 9.67 g/L. Based on these results, this autochthonous strain was applied in bioaugmentation as a bioremediation strategy through microcosm designs, reaching 93.52% of total hydrocarbon removal at 60 days. This constitutes a promising alternative for hydrocarbon-contaminated soil.

Supplementary information

The online version contains supplementary material available at 10.1007/s13205-022-03183-6.

SUBMITTER: Conde Molina D

PROVIDER: S-EPMC9033923 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature

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Optimization of biomass production by autochthonous Pseudomonas sp. MT1A3 as strategy to apply bioremediation in situ in a chronically hydrocarbon-contaminated soil.

Conde Molina Débora D Liporace Franco A FA Quevedo Carla V CV

3 Biotech 20220422 5

These days, petroleum hydrocarbon pollution has become a global problem, because of this, bioremediation is presented as a strategy for cleaning up sites contaminated with organic pollutants, and it has an increasing role in relation to the potential it presents as a non-invasive and cost-effective technology. The aim of this study is to optimize the biomass production of Pseudomonas sp. MT1A3 strain as a soil bioremediation approach for petroleum hydrocarbon polluted environments. Factor ...[more]

PMID: 35547010

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Dataset Information

Optimization of biomass production by autochthonous Pseudomonas sp. MT1A3 as strategy to apply bioremediation in situ in a chronically hydrocarbon-contaminated soil.

Supplementary information

Publications

Optimization of biomass production by autochthonous <i>Pseudomonas</i> sp<i>.</i> MT1A3 as strategy to apply bioremediation in situ in a chronically hydrocarbon-contaminated soil.

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