Thermal desorption effects on fragment ion production from multi-photon ionized uridine and selected analogues.

ABSTRACT: Experiments on neutral gas-phase nucleosides are often complicated by thermal lability. Previous mass spectrometry studies of nucleosides have identified enhanced relative production of nucleobase ions (e.g. uracil⁺ from uridine) as a function of desorption temperature to be the critical indicator of thermal decomposition. On this basis, the present multi-photon ionization (MPI) experiments demonstrate that laser-based thermal desorption is effective for producing uridine, 5-methyluridine, and 2'-deoxyuridine targets without thermal decomposition. Our experiments also revealed one notable thermal dependence: the relative production of the sugar ion C₅H₉O₄ ⁺ from intact uridine increased substantially with the desorption laser po

SUBMITTER: Bockova J 

PROVIDER: S-EPMC9033967 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature