Dihydronicotinamide riboside: synthesis from nicotinamide riboside chloride, purification and stability studies.

ABSTRACT: In the present work, we describe an efficient method for scalable synthesis and purification of 1,4-dihydronicotinamide riboside (NRH) from commercially available nicotinamide riboside chloride (NRCl) and in the presence of sodium dithionate as a reducing agent. NRH is industrially relevant as the most effective, synthetic NAD⁺ precursor. We demonstrated that solid phase synthesis cannot be used for the reduction of NRCl to NRH in high yield, whereas a reduction reaction in water at room temperature under anaerobic conditions is shown to be very effective, reaching a 55% isolation yield. For the first time, by using common column chromatography, we were able to highly purify this sensitive bio-compound with good yield. A series of identifications and analyses including HPLC, NMR

SUBMITTER: Zarei A 

PROVIDER: S-EPMC9034155 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature