Targeted glucocorticoid analysis using ion mobility-mass spectrometry (IM-MS).

ABSTRACT:

Introduction

Ion mobility-mass spectrometry (IM-MS) is an emerging technique in the -omics fields that has broad potential applicability to the clinical lab. As a rapid, gas-phase structure-based separation technique, IM-MS offers promise in isomer separations and can be easily combined with existing LC-MS methods (i.e., LC-IM-MS). Several experimental conditions, including analyte cation adducts and drift composition further provide a means to tune separations for global and/or targeted applications.

Objectives

The primary objective of this study was to demonstrate the utility of IM-MS under a range of experimental conditions for detection of glucocorticoids, and specifically for the separation of several isomeric pairs.

Methods

LC-IM-MS was used to characterize 16 g

SUBMITTER: Neal SP 

PROVIDER: S-EPMC9034309 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature