AuNP array coated substrate for sensitive and homogeneous SERS-immunoassay detection of human immunoglobulin G.

ABSTRACT: Owing to the high sensitivity, fast responsiveness and high specificity, immunoassays using surface-enhanced Raman scattering (SERS) as the readout signal displayed great potential in disease diagnosis. In this study, we developed a SERS-immunoassay method for the detection of human immunoglobulin G (HIgG). Upon involving well-ordered AuA on a SERSIA substrate, the LSPR effect was further enhanced to generate a strong and uniform Raman signal through the formation of sandwich structure with the addition of target HIgG and SERSIA tag. Optimization of the assay provided a wide linear range (0.1-200 μg mL^-1) and low limit of detection (0.1 μg mL^-1). In addition, the SERS-immunoassay method displayed excellent specificity and was homogeneous, which guaranteed the practica

SUBMITTER: Qu Q 

PROVIDER: S-EPMC9034334 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature