Allogeneic haematopoietic stem cell transplantation resets T- and B-cell compartments in sickle cell disease patients.

ABSTRACT:

Objectives

Allogeneic haematopoietic stem cell transplantation (allo-HSCT) is the only currently available curative treatment for sickle cell disease (SCD). Here, we comprehensively evaluated the reconstitution of T- and B-cell compartments in 29 SCD patients treated with allo-HSCT and how it correlated with the development of acute graft-versus-host disease (aGvHD).

Methods

T-cell neogenesis was assessed by quantification of signal-joint and β-chain TCR excision circles. B-cell neogenesis was evaluated by quantification of signal-joint and coding-joint K-chain recombination excision circles. T- and B-cell peripheral subset numbers were assessed by flow cytometry.

Results

Before allo-HSCT (baseline), T-cell neogenesis was normal in SCD patients compared with age-, gen

SUBMITTER: Jarduli-Maciel LR 

PROVIDER: S-EPMC9035210 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature