A barcoding pipeline for mosquito surveillance in Nepal, a biodiverse dengue-endemic country.

ABSTRACT:

Background

Vector-borne diseases are on the rise on a global scale, which is anticipated to further accelerate because of anthropogenic climate change. Resource-limited regions are especially hard hit by this increment with the currently implemented surveillance programs being inadequate for the observed expansion of potential vector species. Cost-effective methods that can be easily implemented in resource-limited settings, e.g. under field conditions, are thus urgently needed to function as an early warning system for vector-borne disease epidemics. Our aim was to enhance entomological capacity in Nepal, a country with endemicity of numerous vector-borne diseases and with frequent outbreaks of dengue fever.

Methods

We used a field barcoding pipeline based on DNA nanopore s

SUBMITTER: Hartke J 

PROVIDER: S-EPMC9035287 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature