CRISPR-Cas9-mediated loss of function of β-catenin attenuates intervertebral disc degeneration.

ABSTRACT: Intervertebral disc degeneration is a very common medical condition causing pain and disability, and it cannot be reversed by available treatment options. Here we report that targeting β-catenin, a pivotal factor associated with disc degeneration, ameliorates disc degeneration in a mouse model of disc injury. Degenerative changes in the disc in response to disc injury include decompression of nucleus pulposus (NP), replacement of notochordal cells in the NP by chondrocyte-like cells, and disorganization of annulus fibrosus (AF). Importantly, downregulation of β-catenin through intradiscal injection of CRISPR-Cas9-expressing adeno-associated virus significantly mitigated all these pathological changes, by preserving notochordal cells and attenuating chondro-osteogenesis in the NP, as well a

SUBMITTER: Fan Y 

PROVIDER: S-EPMC9035381 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature