Comparative Network Biology Discovers Protein Complexes That Underline Cellular Differentiation in Anabaena sp.

ABSTRACT: The filamentous cyanobacterium Anabaena sp. PCC 7120 can differentiate into heterocysts to fix atmospheric nitrogen. During cell differentiation, cellular morphology and gene expression undergo a series of significant changes. To uncover the mechanisms responsible for these alterations, we built protein-protein interaction (PPI) networks for these two cell types by cofractionation coupled with mass spectrometry. We predicted 280 and 215 protein complexes, with 6322 and 2791 high-confidence PPIs in vegetative cells and heterocysts, respectively. Most of the proteins in both types of cells presented similar elution profiles, whereas the elution peaks of 438 proteins showed significant changes. We observed that some well-known complexes recruited new members in heterocysts, such as ribosomes,

SUBMITTER: Xu C 

PROVIDER: S-EPMC9035410 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature