A Triple High Throughput Screening for Extracellular Vesicle Inducing Agents With Immunostimulatory Activity.

ABSTRACT: Extracellular vesicles (EVs) play an important role in intercellular communication and regulation of cells, especially in the immune system where EVs can participate in antigen presentation and may have adjuvant effects. We aimed to identify small molecule compounds that can increase EV release and thereby enhance the immunogenicity of vaccines. We utilized a THP-1 reporter cell line engineered to release EV-associated tetraspanin (CD63)-Turbo-luciferase to quantitatively measure EVs released in culture supernatants as a readout of a high throughput screen (HTS) of 27,895 compounds. In parallel, the cytotoxicity of the compounds was evaluated by PrestoBlue dye assay. For screening immunostimulatory potency, we performed two additional independent HTS on the same compound library using NF-κ

SUBMITTER: Shukla NM 

PROVIDER: S-EPMC9035538 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature