Capsaicin inhibits intestinal Cl^- secretion and promotes Na⁺ absorption by blocking TRPV4 channels in healthy and colitic mice.

ABSTRACT: Although capsaicin has been studied extensively as an activator of the transient receptor potential vanilloid cation channel subtype 1 (TRPV1) channels in sensory neurons, little is known about its TRPV1-independent actions in gastrointestinal health and disease. Here, we aimed to investigate the pharmacological actions of capsaicin as a food additive and medication on intestinal ion transporters in mouse models of ulcerative colitis (UC). The short-circuit current (I_sc) of the intestine from WT, TRPV1-, and TRPV4-KO mice were measured in Ussing chambers, and Ca²⁺ imaging was performed on small intestinal epithelial cells. We also performed Western blots, immunohistochemistry, and immunofluorescence on intestinal epithelial cells and on intestinal tissues following UC

SUBMITTER: Wan H 

PROVIDER: S-EPMC9035713 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature