Spatiotemporal three-dimensional transport dynamics of endocytic cargos and their physical regulations in cells.

ABSTRACT: Intracellular transport, regulated by complex cytoarchitectures and active driving forces, is crucial for biomolecule translocations and relates to many cellular functions. Despite extensive knowledge obtained from two-dimensional (2D) experiments, the real three-dimensional (3D) spatiotemporal characteristics of intracellular transport is still unclear. With 3D single-particle tracking, we comprehensively studied the transport dynamics of endocytic cargos. With varying timescale, the intracellular transport changes from thermal-dominated 3D-constrained motion to active-dominated quasi-2D motion. Spatially, the lateral motion is heterogeneous with peripheral regions being faster than perinuclear regions, while the axial motion is homogeneous across the cells. We further confirmed that such

SUBMITTER: Jiang C 

PROVIDER: S-EPMC9035719 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature